The protein encoded by this gene is a peroxisomal thioesterase that appears to be involved more in the oxidation of fatty acids rather than in their formation. The encoded protein can bind to the human immunodeficiency virus-1 protein Nef, and mediate Nef-induced down-regulation of CD4 in T-cells. [provided by RefSeq, Oct 2010]
Transcription factors with Perturb-seq knockdown data for ACOT8. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ACOT8 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ACOT8, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr20:45,764,655–45,765,666 | 92.3 kb | Distal (>10kb) Multiome | 192 | |
| chr20:45,770,709–45,771,657 | 86.2 kb | Distal (>10kb) Multiome | 436 | |
| chr20:45,791,633–45,792,259 | 65.5 kb | Distal (>10kb) Multiome | 882 | |
| chr20:45,812,087–45,813,052 | 44.9 kb | Distal (>10kb) Multiome | 695 | |
| chr20:45,833,493–45,834,622 | 23.4 kb | Distal (>10kb) Multiome | 833 | |
| chr20:45,854,794–45,855,232 | 2.2 kb | Proximal (<10kb) | 28 | |
| chr20:45,856,871–45,858,137 | 126 bp | At TSS Multiome | 884 | |
| chr20:45,880,796–45,881,524 | 23.8 kb | Distal (>10kb) Multiome | 738 | |
| chr20:45,890,127–45,891,800 | 34.0 kb | Distal (>10kb) Multiome | 1095 | |
| chr20:45,910,538–45,912,397 | 54.9 kb | Distal (>10kb) Multiome | 713 | |
| chr20:45,933,591–45,935,780 | 77.7 kb | Distal (>10kb) Multiome | 1195 | |
| chr20:45,971,553–45,972,790 | 114.8 kb | Distal (>10kb) Multiome | 1206 | |
| chr20:46,004,647–46,005,315 | 147.6 kb | Distal (>10kb) Multiome | 207 | |
| chr20:46,011,604–46,012,166 | 154.5 kb | Distal (>10kb) Multiome | 239 | |
| chr20:46,013,565–46,014,586 | 156.7 kb | Distal (>10kb) Multiome | 735 | |
| chr20:46,021,209–46,023,166 | 164.6 kb | Distal (>10kb) Multiome | 679 | |
| chr20:46,031,750–46,032,261 | 174.6 kb | Distal (>10kb) Multiome | 227 | |
| chr20:46,058,408–46,058,983 | 201.4 kb | Distal (>10kb) Multiome | 123 | |
| chr20:46,089,092–46,090,355 | 232.5 kb | Distal (>10kb) Multiome | 1047 | |
| chr20:46,091,736–46,092,300 | 234.8 kb | Distal (>10kb) Multiome | 38 | |
| chr20:46,117,897–46,118,755 | 260.9 kb | Distal (>10kb) Multiome | 376 |
Genomic view of the ACOT8 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.