This gene encodes a protein that is highly similar to mouse and rat kynurenine aminotransferase II. The rat protein is a homodimer with two transaminase activities. One activity is the transamination of alpha-aminoadipic acid, a final step in the saccaropine pathway which is the major pathway for L-lysine catabolism. The other activity involves the transamination of kynurenine to produce kynurenine acid, the precursor of kynurenic acid which has neuroprotective properties. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]
Transcription factors with Perturb-seq knockdown data for AADAT. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = AADAT upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of AADAT, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr4:169,774,514–169,775,438 | 315.2 kb | Distal (>10kb) Multiome HiCAR | 195 | |
| chr4:169,918,815–169,920,220 | 170.3 kb | Distal (>10kb) Multiome | 227 | |
| chr4:169,934,787–169,935,471 | 155.2 kb | Distal (>10kb) Multiome | 121 | |
| chr4:170,004,428–170,005,171 | 85.5 kb | Distal (>10kb) Multiome | 112 | |
| chr4:170,025,638–170,027,809 | 63.8 kb | Distal (>10kb) Multiome | 968 | |
| chr4:170,088,782–170,088,930 | 1.3 kb | Proximal (<10kb) | 25 | |
| chr4:170,089,100–170,090,754 | 74 bp | At TSS Multiome | 761 | |
| chr4:170,090,855–170,092,203 | 1.4 kb | Proximal (<10kb) Multiome | 192 | |
| chr4:170,109,620–170,111,763 | 20.3 kb | Distal (>10kb) Multiome | 267 | |
| chr4:170,259,234–170,260,354 | 169.6 kb | Distal (>10kb) Multiome | 326 | |
| chr4:170,285,465–170,286,907 | 195.8 kb | Distal (>10kb) Multiome | 211 |
Genomic view of the AADAT locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.