Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for ZYX. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ZYX upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ZYX, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr7:143,263,111–143,264,043 | 117.8 kb | Distal (>10kb) Multiome | 857 | |
| chr7:143,284,394–143,285,772 | 96.4 kb | Distal (>10kb) Multiome | 381 | |
| chr7:143,287,670–143,288,978 | 93.0 kb | Distal (>10kb) Multiome | 878 | |
| chr7:143,345,279–143,346,282 | 35.7 kb | Distal (>10kb) Multiome | 433 | |
| chr7:143,361,157–143,363,235 | 18.5 kb | Distal (>10kb) Multiome | 388 | |
| chr7:143,380,012–143,382,328 | 139 bp | At TSS Multiome | 946 | |
| chr7:143,387,556–143,387,961 | 6.3 kb | Proximal (<10kb) | 115 | |
| chr7:143,388,345–143,388,991 | 7.1 kb | Proximal (<10kb) | 77 | |
| chr7:143,389,538–143,390,929 | 8.2 kb | Proximal (<10kb) | 117 | |
| chr7:143,405,536–143,406,754 | 24.9 kb | Distal (>10kb) Multiome HiCAR | 194 | |
| chr7:143,407,435–143,409,651 | 27.6 kb | Distal (>10kb) Multiome | 459 |
Genomic view of the ZYX locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.