This gene encodes an E3 ubiquitin-protein ligase that plays a role in neural-cell differentiation. Overexpression of this gene causes neurite-like elongation. The encoded protein contains both a zinc finger and a RING finger motif and is localized in the endosome/lysosome compartment, indicating that it may be involved in ubiquitin-mediated protein modification, and in synaptic vessicle membranes in neurons. [provided by RefSeq, Feb 2012]
Transcription factors with Perturb-seq knockdown data for ZNRF1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ZNRF1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ZNRF1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr16:74,700,393–74,701,030 | 298.4 kb | Distal (>10kb) Multiome | 785 | |
| chr16:74,774,151–74,775,199 | 224.2 kb | Distal (>10kb) Multiome | 669 | |
| chr16:74,812,990–74,813,481 | 185.7 kb | Distal (>10kb) Multiome | 680 | |
| chr16:74,866,003–74,866,478 | 132.8 kb | Distal (>10kb) Multiome | 95 | |
| chr16:74,984,580–74,985,562 | 13.9 kb | Distal (>10kb) Multiome | 877 | |
| chr16:74,998,533–75,000,404 | 87 bp | At TSS Multiome | 884 | |
| chr16:75,000,687–75,000,841 | 1.7 kb | Proximal (<10kb) | 176 | |
| chr16:75,003,493–75,003,763 | 4.5 kb | Proximal (<10kb) | 194 | |
| chr16:75,110,910–75,111,744 | 112.3 kb | Distal (>10kb) Multiome HiCAR | 720 | |
| chr16:75,148,292–75,149,298 | 149.6 kb | Distal (>10kb) Multiome | 970 | |
| chr16:75,250,387–75,252,500 | 252.7 kb | Distal (>10kb) Multiome | 811 | |
| chr16:75,265,114–75,266,526 | 267.0 kb | Distal (>10kb) Multiome | 798 |
Genomic view of the ZNRF1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.