Predicted to enable DNA binding activity and zinc ion binding activity. Predicted to be involved in regulation of transcription by RNA polymerase II. Predicted to be active in nucleus. [provided by Alliance of Genome Resources, Jul 2025]
Modules significantly affected by knockdown. ↑ Up = module upregulated upon KD; ↓ Down = module downregulated upon KD.
| Cluster | Dir | NES | padj | Bind | OR | padj (bind) |
|---|
| Module | Dir | NES | #gRNA | padj | Bind | OR | padj (bind) |
|---|
| Submodule | Module | Dir | NES | #gRNA | Bind | OR | padj (bind) |
|---|
Genes likely regulated by ZNF850 through linked binding evidence in open chromatin. The chart ranks TF-linked genes by their mean Perturb-seq response to ZNF850 knockdown, with negative coefficients indicating downregulation and positive coefficients indicating upregulation upon knockdown.
Open chromatin elements (ATAC-seq) where ZNF850 has ChIP-seq or motif footprint binding evidence and which are linked to at least one target gene region.
| Element | Size | Linked genes |
|---|
Transcription factors with Perturb-seq knockdown data for ZNF850. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ZNF850 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ZNF850, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr19:36,488,856–36,490,274 | 283.2 kb | Distal (>10kb) Multiome | 991 | |
| chr19:36,527,962–36,529,500 | 244.3 kb | Distal (>10kb) Multiome | 937 | |
| chr19:36,572,622–36,573,936 | 199.5 kb | Distal (>10kb) Multiome | 1017 | |
| chr19:36,604,710–36,605,744 | 167.5 kb | Distal (>10kb) Multiome | 657 | |
| chr19:36,666,500–36,667,489 | 105.9 kb | Distal (>10kb) Multiome | 842 | |
| chr19:36,686,842–36,688,044 | 85.3 kb | Distal (>10kb) Multiome | 876 | |
| chr19:36,771,571–36,773,224 | 1.1 kb | Proximal (<10kb) Multiome | 721 | |
| chr19:36,837,900–36,839,014 | 65.6 kb | Distal (>10kb) Multiome | 855 | |
| chr19:36,849,748–36,851,333 | 77.5 kb | Distal (>10kb) Multiome | 755 | |
| chr19:36,915,949–36,916,617 | 143.5 kb | Distal (>10kb) Multiome | 584 | |
| chr19:36,973,037–36,973,876 | 200.6 kb | Distal (>10kb) Multiome | 240 |
Genomic view of the ZNF850 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.