Transcription factors with Perturb-seq knockdown data for ZNF528-AS1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ZNF528-AS1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ZNF528-AS1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr19:52,139,087–52,140,222 | 257.8 kb | Distal (>10kb) Multiome | 788 | |
| chr19:52,170,935–52,172,031 | 226.2 kb | Distal (>10kb) Multiome | 404 | |
| chr19:52,189,175–52,190,788 | 207.8 kb | Distal (>10kb) Multiome | 912 | |
| chr19:52,269,012–52,270,193 | 128.3 kb | Distal (>10kb) Multiome | 782 | |
| chr19:52,296,552–52,297,775 | 100.6 kb | Distal (>10kb) Multiome | 561 | |
| chr19:52,335,886–52,336,825 | 61.6 kb | Distal (>10kb) Multiome | 373 | |
| chr19:52,338,866–52,339,647 | 58.5 kb | Distal (>10kb) Multiome | 56 | |
| chr19:52,492,205–52,493,529 | 95.1 kb | Distal (>10kb) Multiome | 178 | |
| chr19:52,526,868–52,528,322 | 129.7 kb | Distal (>10kb) Multiome | 602 | |
| chr19:52,535,433–52,536,654 | 138.2 kb | Distal (>10kb) Multiome | 273 | |
| chr19:52,558,780–52,559,576 | 161.4 kb | Distal (>10kb) Multiome | 17 | |
| chr19:52,569,672–52,570,898 | 172.4 kb | Distal (>10kb) Multiome | 555 | |
| chr19:52,600,732–52,601,970 | 203.4 kb | Distal (>10kb) Multiome | 412 | |
| chr19:52,637,710–52,638,958 | 240.6 kb | Distal (>10kb) Multiome | 473 | |
| chr19:52,690,252–52,691,142 | 292.8 kb | Distal (>10kb) Multiome | 540 |
Genomic view of the ZNF528-AS1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.