Pre-mRNA splicing is catalyzed by the spliceosome. U12-type spliceosome binds U12-type pre-mRNAs and recognizes the 5' splice site and branch-point sequence. U11 and U12 snRNPs are components of U12-type spliceosome and function as a molecular bridge connecting both ends of the intron. The protein encoded by this gene contains a RNA recognition motif. It was identified as one of the protein components of U11/U12 snRNPs. This protein and many other U11/U12 snRNP proteins are highly conserved in organisms known to contain U12-type introns. These proteins have been shown to be essential for cell viability, suggesting the key roles in U12-type splicing. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for ZCRB1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ZCRB1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ZCRB1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:40,692,027–40,693,873 | 1633.7 kb | Distal (>10kb) Multiome HiCAR | 487 | |
| chr12:40,745,253–40,745,981 | 1580.5 kb | Distal (>10kb) Multiome HiCAR | 73 | |
| chr12:42,144,190–42,146,161 | 181.3 kb | Distal (>10kb) Multiome HiCAR | 923 | |
| chr12:42,237,254–42,239,022 | 88.5 kb | Distal (>10kb) Multiome | 833 | |
| chr12:42,325,595–42,326,457 | 74 bp | At TSS Multiome | 748 | |
| chr12:42,482,483–42,484,824 | 158.0 kb | Distal (>10kb) Multiome | 761 | |
| chr12:42,588,923–42,590,912 | 263.7 kb | Distal (>10kb) Multiome | 540 |
Genomic view of the ZCRB1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.