This gene encodes a scaffold protein which serves as an assessory factor to the nuclear RNA exosome complex. The encoded protein forms a trimeric human nuclear exosome targeting (NEXT) complex, together with hMTR4 and the RNA-binding factor RBM7 which promotes the exosomal degradation of non-coding promoter-upstream transcripts, enhancer RNAs and 3'-extended products of histone- and small nuclear RNA transcription. This complex is also thought to recruit the exosome to degrade intronic RNAs via its interaction with both the exosome and the spliceosome. It contains both an N-terminal zinc-knuckle domain and a C-terminal proline-rich domain. [provided by RefSeq, Apr 2017]
Transcription factors with Perturb-seq knockdown data for ZCCHC8. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ZCCHC8 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ZCCHC8, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:122,203,186–122,204,669 | 297.3 kb | Distal (>10kb) Multiome | 500 | |
| chr12:122,225,485–122,227,116 | 274.7 kb | Distal (>10kb) Multiome | 1019 | |
| chr12:122,265,933–122,267,109 | 234.4 kb | Distal (>10kb) Multiome | 1060 | |
| chr12:122,421,844–122,422,754 | 78.5 kb | Distal (>10kb) Multiome | 685 | |
| chr12:122,500,206–122,501,493 | 60 bp | At TSS Multiome | 955 | |
| chr12:122,526,435–122,527,753 | 26.1 kb | Distal (>10kb) Multiome | 956 | |
| chr12:122,751,194–122,751,767 | 250.4 kb | Distal (>10kb) Multiome | 128 | |
| chr12:122,752,332–122,753,299 | 251.8 kb | Distal (>10kb) Multiome | 978 | |
| chr12:122,774,223–122,774,865 | 273.6 kb | Distal (>10kb) Multiome | 445 |
Genomic view of the ZCCHC8 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.