Transcription factors with Perturb-seq knockdown data for WNT5A-AS1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = WNT5A-AS1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of WNT5A-AS1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr3:55,481,937–55,483,316 | 4.4 kb | Proximal (<10kb) | 116 | |
| chr3:55,484,296–55,484,639 | 3.1 kb | Proximal (<10kb) | 44 | |
| chr3:55,484,722–55,485,839 | 1.9 kb | Proximal (<10kb) | 348 | |
| chr3:55,486,105–55,488,985 | at TSS | At TSS | 509 | |
| chr3:55,489,460–55,489,744 | 1.8 kb | Proximal (<10kb) | 72 | |
| chr3:55,489,853–55,490,182 | 2.2 kb | Proximal (<10kb) | 125 |
Genomic view of the WNT5A-AS1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.