This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. Two patients with Sensenbrenner syndrome / cranioectodermal dysplasia (CED) were identified with mutations in this gene, consistent with a possible ciliary function.[provided by RefSeq, Sep 2010]
Transcription factors with Perturb-seq knockdown data for WDR35. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = WDR35 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of WDR35, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr2:19,863,073–19,864,223 | 126.5 kb | Distal (>10kb) Multiome | 720 | |
| chr2:19,868,540–19,869,572 | 121.2 kb | Distal (>10kb) Multiome | 302 | |
| chr2:19,901,191–19,902,460 | 88.3 kb | Distal (>10kb) Multiome | 1066 | |
| chr2:19,989,520–19,990,653 | 42 bp | At TSS Multiome | 924 | |
| chr2:20,011,873–20,013,148 | 22.5 kb | Distal (>10kb) Multiome | 585 | |
| chr2:20,050,661–20,052,578 | 61.8 kb | Distal (>10kb) Multiome | 1029 | |
| chr2:20,097,914–20,098,555 | 108.3 kb | Distal (>10kb) Multiome | 480 | |
| chr2:20,106,361–20,107,468 | 116.8 kb | Distal (>10kb) Multiome | 181 | |
| chr2:20,207,998–20,208,855 | 218.3 kb | Distal (>10kb) Multiome | 346 | |
| chr2:20,223,290–20,226,136 | 233.6 kb | Distal (>10kb) Multiome | 1023 | |
| chr2:20,242,059–20,243,494 | 252.4 kb | Distal (>10kb) Multiome | 372 |
Genomic view of the WDR35 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.