The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this protein plays a role in DNA postreplication repair. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for UBE2N. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = UBE2N upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of UBE2N, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:93,245,335–93,245,870 | 196.3 kb | Distal (>10kb) Multiome | 155 | |
| chr12:93,376,338–93,378,831 | 64.1 kb | Distal (>10kb) Multiome | 827 | |
| chr12:93,441,175–93,442,781 | 141 bp | At TSS Multiome | 926 | |
| chr12:93,467,156–93,468,248 | 25.6 kb | Distal (>10kb) Multiome | 844 | |
| chr12:93,569,457–93,573,628 | 129.0 kb | Distal (>10kb) Multiome | 1052 | |
| chr12:93,677,025–93,678,145 | 235.5 kb | Distal (>10kb) Multiome | 999 | |
| chr12:93,741,675–93,742,916 | 300.2 kb | Distal (>10kb) Multiome | 726 |
Genomic view of the UBE2N locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.