The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]
Transcription factors with Perturb-seq knockdown data for UBE2D1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = UBE2D1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of UBE2D1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr10:58,050,151–58,051,097 | 284.3 kb | Distal (>10kb) Multiome | 130 | |
| chr10:58,140,507–58,141,288 | 194.0 kb | Distal (>10kb) Multiome | 122 | |
| chr10:58,266,789–58,269,963 | 67.1 kb | Distal (>10kb) Multiome | 1224 | |
| chr10:58,334,319–58,334,511 | 466 bp | At TSS | 7 | |
| chr10:58,334,610–58,335,934 | 33 bp | At TSS Multiome | 801 | |
| chr10:58,384,591–58,386,114 | 50.4 kb | Distal (>10kb) Multiome | 1000 | |
| chr10:58,512,205–58,513,965 | 178.0 kb | Distal (>10kb) Multiome | 523 | |
| chr10:58,576,755–58,577,603 | 242.2 kb | Distal (>10kb) Multiome | 159 |
Genomic view of the UBE2D1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.