Enables several functions, including iron ion binding activity; protein homodimerization activity; and tRNA(Phe) (7-(3-amino-3-carboxypropyl)wyosine37-C2)-hydroxylase activity. Involved in wybutosine biosynthetic process. Predicted to be located in cytoplasm. [provided by Alliance of Genome Resources, Jul 2025]
Transcription factors with Perturb-seq knockdown data for TYW5. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TYW5 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TYW5, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr2:199,850,490–199,851,605 | 104.4 kb | Distal (>10kb) Multiome | 817 | |
| chr2:199,910,668–199,912,042 | 44.2 kb | Distal (>10kb) Multiome | 929 | |
| chr2:199,953,877–199,954,033 | 1.5 kb | Proximal (<10kb) | 17 | |
| chr2:199,955,094–199,956,570 | 84 bp | At TSS Multiome | 1078 | |
| chr2:200,090,238–200,091,621 | 135.8 kb | Distal (>10kb) Multiome | 66 | |
| chr2:200,201,704–200,202,415 | 246.5 kb | Distal (>10kb) Multiome | 128 | |
| chr2:200,203,430–200,204,082 | 248.2 kb | Distal (>10kb) Multiome | 141 | |
| chr2:200,481,420–200,481,868 | 526.1 kb | Distal (>10kb) Multiome HiCAR | 350 | |
| chr2:200,509,374–200,510,438 | 554.6 kb | Distal (>10kb) Multiome HiCAR | 876 | |
| chr2:200,525,770–200,526,701 | 570.6 kb | Distal (>10kb) Multiome HiCAR | 869 | |
| chr2:200,585,716–200,586,842 | 630.6 kb | Distal (>10kb) Multiome HiCAR | 627 | |
| chr2:200,713,048–200,713,472 | 757.8 kb | Distal (>10kb) Multiome HiCAR | 763 | |
| chr2:200,810,854–200,812,782 | 856.1 kb | Distal (>10kb) Multiome HiCAR | 1048 |
Genomic view of the TYW5 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.