TTL is a cytosolic enzyme involved in the posttranslational modification of alpha-tubulin (see MIM 602529). Alpha-tubulin within assembled microtubules is detyrosinated over time at the C terminus. After microtubule disassembly, TTL restores the tyrosine residues and consequently participates in a cycle of tubulin detyrosination and tyrosination (Erck et al., 2003 [PubMed 14571137]).[supplied by OMIM, Mar 2008]
Transcription factors with Perturb-seq knockdown data for TTL. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TTL upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TTL, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr2:112,181,502–112,182,275 | 300.3 kb | Distal (>10kb) Multiome | 174 | |
| chr2:112,254,532–112,255,517 | 227.1 kb | Distal (>10kb) Multiome | 915 | |
| chr2:112,275,156–112,276,567 | 205.9 kb | Distal (>10kb) Multiome | 1201 | |
| chr2:112,480,926–112,482,608 | 1.0 kb | Proximal (<10kb) Multiome | 860 | |
| chr2:112,541,785–112,542,849 | 60.1 kb | Distal (>10kb) Multiome | 1070 | |
| chr2:112,583,940–112,585,172 | 102.4 kb | Distal (>10kb) Multiome | 949 | |
| chr2:112,645,080–112,646,681 | 163.6 kb | Distal (>10kb) Multiome | 980 | |
| chr2:112,674,793–112,675,390 | 192.9 kb | Distal (>10kb) Multiome | 54 | |
| chr2:112,726,233–112,727,218 | 244.7 kb | Distal (>10kb) Multiome | 667 | |
| chr2:112,763,867–112,765,097 | 282.6 kb | Distal (>10kb) Multiome | 854 |
Genomic view of the TTL locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.