Transcription factors with Perturb-seq knockdown data for TTC33. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TTC33 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TTC33, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr5:40,678,872–40,680,670 | 76.1 kb | Distal (>10kb) Multiome | 615 | |
| chr5:40,680,710–40,682,280 | 74.3 kb | Distal (>10kb) Multiome | 343 | |
| chr5:40,754,888–40,756,381 | 14 bp | At TSS Multiome | 1007 | |
| chr5:40,797,344–40,798,944 | 42.3 kb | Distal (>10kb) Multiome | 949 | |
| chr5:40,834,732–40,836,235 | 79.4 kb | Distal (>10kb) Multiome | 1118 | |
| chr5:41,509,222–41,511,087 | 754.7 kb | Distal (>10kb) Multiome HiCAR | 331 |
Genomic view of the TTC33 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.