Transcription factors with Perturb-seq knockdown data for TTC27. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TTC27 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TTC27, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr2:32,356,410–32,358,403 | 271.1 kb | Distal (>10kb) Multiome | 1140 | |
| chr2:32,381,392–32,382,477 | 246.4 kb | Distal (>10kb) Multiome | 98 | |
| chr2:32,619,915–32,620,253 | 7.8 kb | Proximal (<10kb) | 27 | |
| chr2:32,626,481–32,627,181 | 913 bp | At TSS | 11 | |
| chr2:32,627,287–32,628,586 | 93 bp | At TSS Multiome | 825 |
Genomic view of the TTC27 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.