The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. Alternatively spliced transcripts encoding the same protein have been identified. [provided by RefSeq, Nov 2009]
Transcription factors with Perturb-seq knockdown data for TSPAN9. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TSPAN9 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TSPAN9, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:2,794,243–2,796,266 | 281.4 kb | Distal (>10kb) Multiome | 802 | |
| chr12:2,812,386–2,813,317 | 264.7 kb | Distal (>10kb) Multiome | 1037 | |
| chr12:2,845,941–2,846,561 | 231.2 kb | Distal (>10kb) Multiome | 220 | |
| chr12:2,876,673–2,877,899 | 200.2 kb | Distal (>10kb) Multiome | 809 | |
| chr12:2,890,285–2,891,599 | 186.6 kb | Distal (>10kb) Multiome | 880 | |
| chr12:2,958,171–2,960,222 | 118.1 kb | Distal (>10kb) Multiome | 770 | |
| chr12:3,073,483–3,073,679 | 3.7 kb | Proximal (<10kb) | 227 | |
| chr12:3,076,742–3,077,982 | 130 bp | At TSS Multiome | 614 | |
| chr12:3,172,314–3,172,997 | 95.3 kb | Distal (>10kb) Multiome | 53 | |
| chr12:3,199,420–3,200,898 | 122.8 kb | Distal (>10kb) Multiome | 348 | |
| chr12:3,214,367–3,215,060 | 137.3 kb | Distal (>10kb) Multiome | 142 | |
| chr12:3,365,831–3,366,469 | 288.8 kb | Distal (>10kb) Multiome | 149 |
Genomic view of the TSPAN9 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.