The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The use of alternate polyadenylation sites has been found for this gene. Multiple alternative transcripts encoding different isoforms have been described. [provided by RefSeq, Dec 2009]
Transcription factors with Perturb-seq knockdown data for TSPAN3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TSPAN3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TSPAN3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr15:76,904,474–76,906,082 | 165.7 kb | Distal (>10kb) Multiome | 1003 | |
| chr15:76,931,243–76,932,242 | 139.5 kb | Distal (>10kb) Multiome | 693 | |
| chr15:76,973,226–76,973,843 | 97.6 kb | Distal (>10kb) Multiome | 182 | |
| chr15:77,027,800–77,028,685 | 42.8 kb | Distal (>10kb) Multiome | 156 | |
| chr15:77,070,415–77,071,533 | 31 bp | At TSS Multiome | 652 | |
| chr15:77,083,772–77,084,179 | at TSS | At TSS | 289 | |
| chr15:77,525,254–77,525,853 | 454.4 kb | Distal (>10kb) Multiome HiCAR | 43 | |
| chr15:77,528,312–77,529,264 | 457.8 kb | Distal (>10kb) Multiome HiCAR | 100 | |
| chr15:77,543,155–77,543,857 | 472.4 kb | Distal (>10kb) Multiome HiCAR | 680 | |
| chr15:77,604,253–77,604,752 | 533.4 kb | Distal (>10kb) Multiome HiCAR | 145 | |
| chr15:77,630,922–77,631,706 | 560.2 kb | Distal (>10kb) Multiome HiCAR | 92 | |
| chr15:77,632,252–77,635,007 | 562.6 kb | Distal (>10kb) Multiome HiCAR | 831 |
Genomic view of the TSPAN3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.