This gene encodes a member of the tropomyosin family of actin-binding proteins. Tropomyosins are dimers of coiled-coil proteins that provide stability to actin filaments and regulate access of other actin-binding proteins. Mutations in this gene result in autosomal dominant nemaline myopathy and other muscle disorders. This locus is involved in translocations with other loci, including anaplastic lymphoma receptor tyrosine kinase (ALK) and neurotrophic tyrosine kinase receptor type 1 (NTRK1), which result in the formation of fusion proteins that act as oncogenes. There are numerous pseudogenes for this gene on different chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]
Transcription factors with Perturb-seq knockdown data for TPM3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TPM3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TPM3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:153,922,213–153,923,779 | 260.1 kb | Distal (>10kb) Multiome | 856 | |
| chr1:153,945,717–153,947,118 | 236.5 kb | Distal (>10kb) Multiome | 866 | |
| chr1:153,957,828–153,959,103 | 224.5 kb | Distal (>10kb) Multiome | 912 | |
| chr1:153,963,107–153,964,407 | 219.6 kb | Distal (>10kb) Multiome | 798 | |
| chr1:153,967,147–153,968,324 | 215.6 kb | Distal (>10kb) Multiome | 986 | |
| chr1:153,977,057–153,978,034 | 205.6 kb | Distal (>10kb) Multiome | 853 | |
| chr1:153,985,902–153,987,184 | 196.8 kb | Distal (>10kb) Multiome | 572 | |
| chr1:153,990,402–153,991,082 | 192.5 kb | Distal (>10kb) Multiome | 950 | |
| chr1:154,178,974–154,179,446 | 3.8 kb | Proximal (<10kb) | 158 | |
| chr1:154,180,254–154,180,700 | 2.5 kb | Proximal (<10kb) | 124 | |
| chr1:154,182,339–154,183,774 | 119 bp | At TSS Multiome | 934 | |
| chr1:154,186,183–154,186,551 | 3.0 kb | Proximal (<10kb) | 188 | |
| chr1:154,220,079–154,221,864 | 37.5 kb | Distal (>10kb) Multiome | 1071 | |
| chr1:154,272,082–154,272,847 | 89.3 kb | Distal (>10kb) Multiome | 834 | |
| chr1:154,324,640–154,326,731 | 141.6 kb | Distal (>10kb) Multiome | 685 | |
| chr1:154,328,267–154,328,890 | 145.4 kb | Distal (>10kb) Multiome | 261 | |
| chr1:154,352,626–154,353,487 | 170.0 kb | Distal (>10kb) Multiome | 466 | |
| chr1:154,404,987–154,406,067 | 222.2 kb | Distal (>10kb) Multiome | 731 |
Genomic view of the TPM3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.