Transcription factors with Perturb-seq knockdown data for TMEM254-AS1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TMEM254-AS1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TMEM254-AS1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr10:79,904,553–79,905,313 | 174.0 kb | Distal (>10kb) Multiome | 317 | |
| chr10:79,981,449–79,982,593 | 96.9 kb | Distal (>10kb) Multiome | 420 | |
| chr10:80,070,172–80,070,399 | 8.5 kb | Proximal (<10kb) | 13 | |
| chr10:80,076,334–80,077,082 | 2.3 kb | Proximal (<10kb) Multiome | 10 | |
| chr10:80,078,334–80,079,521 | 65 bp | At TSS Multiome | 806 | |
| chr10:80,132,279–80,133,011 | 53.7 kb | Distal (>10kb) Multiome | 155 | |
| chr10:80,204,736–80,206,157 | 126.7 kb | Distal (>10kb) Multiome | 830 | |
| chr10:80,207,076–80,207,999 | 128.6 kb | Distal (>10kb) Multiome | 466 | |
| chr10:80,265,707–80,266,498 | 187.2 kb | Distal (>10kb) Multiome | 166 | |
| chr10:80,356,265–80,357,947 | 278.0 kb | Distal (>10kb) Multiome | 316 |
Genomic view of the TMEM254-AS1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.