The protein encoded by this gene is similar in sequence to the amino terminus of Drosophila enhancer of split groucho, a protein involved in neurogenesis during embryonic development. The encoded protein, which belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members to dominantly repress the expression of other family member genes. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for TLE5. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TLE5 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TLE5, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr19:2,782,866–2,783,778 | 279.0 kb | Distal (>10kb) Multiome | 998 | |
| chr19:2,784,850–2,785,981 | 277.0 kb | Distal (>10kb) Multiome | 682 | |
| chr19:2,819,641–2,820,625 | 242.5 kb | Distal (>10kb) Multiome | 826 | |
| chr19:2,840,664–2,842,136 | 221.1 kb | Distal (>10kb) Multiome | 903 | |
| chr19:2,900,564–2,901,033 | 161.6 kb | Distal (>10kb) Multiome | 686 | |
| chr19:2,944,631–2,945,468 | 117.4 kb | Distal (>10kb) Multiome | 891 | |
| chr19:2,950,070–2,951,291 | 111.6 kb | Distal (>10kb) Multiome | 962 | |
| chr19:2,976,688–2,977,848 | 85.0 kb | Distal (>10kb) Multiome | 715 | |
| chr19:2,983,511–2,984,586 | 78.3 kb | Distal (>10kb) Multiome | 523 | |
| chr19:3,024,668–3,025,742 | 37.2 kb | Distal (>10kb) Multiome | 159 | |
| chr19:3,035,442–3,037,057 | 26.0 kb | Distal (>10kb) Multiome | 947 | |
| chr19:3,041,823–3,042,438 | 20.2 kb | Distal (>10kb) Multiome | 173 | |
| chr19:3,045,731–3,048,029 | 16.4 kb | Distal (>10kb) Multiome | 612 | |
| chr19:3,062,260–3,062,861 | 150 bp | At TSS Multiome | 369 | |
| chr19:3,093,786–3,095,227 | 31.8 kb | Distal (>10kb) Multiome | 515 | |
| chr19:3,095,637–3,096,163 | 33.6 kb | Distal (>10kb) Multiome | 256 | |
| chr19:3,185,437–3,186,577 | 123.3 kb | Distal (>10kb) Multiome | 751 | |
| chr19:3,224,482–3,224,897 | 162.2 kb | Distal (>10kb) Multiome | 253 | |
| chr19:3,275,036–3,277,129 | 213.9 kb | Distal (>10kb) Multiome | 737 | |
| chr19:3,284,276–3,286,396 | 223.2 kb | Distal (>10kb) Multiome | 555 | |
| chr19:3,360,114–3,360,563 | 297.9 kb | Distal (>10kb) Multiome | 430 |
Genomic view of the TLE5 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.