The innate immune system recognizes microbial pathogens through Toll-like receptors (TLRs), which identify pathogen-associated molecular patterns. Different TLRs recognize different pathogen-associated molecular patterns and all TLRs have a Toll-interleukin 1 receptor (TIR) domain, which is responsible for signal transduction. The protein encoded by this gene is a TIR adaptor protein involved in the TLR4 signaling pathway of the immune system. It activates NF-kappa-B, MAPK1, MAPK3 and JNK, which then results in cytokine secretion and the inflammatory response. Alternative splicing of this gene results in several transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for TIRAP. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TIRAP upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TIRAP, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr11:126,061,736–126,063,712 | 219.4 kb | Distal (>10kb) Multiome | 802 | |
| chr11:126,082,403–126,083,132 | 199.8 kb | Distal (>10kb) Multiome | 134 | |
| chr11:126,104,267–126,104,892 | 177.9 kb | Distal (>10kb) Multiome | 192 | |
| chr11:126,159,698–126,160,582 | 122.4 kb | Distal (>10kb) Multiome | 281 | |
| chr11:126,210,869–126,212,282 | 70.9 kb | Distal (>10kb) Multiome | 846 | |
| chr11:126,268,478–126,269,613 | 13.5 kb | Distal (>10kb) Multiome | 828 | |
| chr11:126,282,310–126,283,787 | 544 bp | At TSS Multiome | 754 | |
| chr11:126,303,694–126,304,639 | 21.6 kb | Distal (>10kb) Multiome | 979 | |
| chr11:126,345,823–126,346,956 | 63.8 kb | Distal (>10kb) Multiome | 446 | |
| chr11:126,355,100–126,356,405 | 73.2 kb | Distal (>10kb) Multiome | 596 | |
| chr11:126,440,475–126,441,596 | 158.5 kb | Distal (>10kb) Multiome | 367 | |
| chr11:126,480,538–126,481,044 | 198.3 kb | Distal (>10kb) Multiome | 487 | |
| chr11:126,497,641–126,499,015 | 216.0 kb | Distal (>10kb) Multiome | 250 | |
| chr11:126,502,215–126,503,760 | 220.7 kb | Distal (>10kb) Multiome | 129 |
Genomic view of the TIRAP locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.