The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for TDG. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TDG upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TDG, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:103,825,918–103,826,568 | 139.5 kb | Distal (>10kb) Multiome | 82 | |
| chr12:103,840,241–103,842,325 | 124.5 kb | Distal (>10kb) Multiome | 911 | |
| chr12:103,928,943–103,931,279 | 35.6 kb | Distal (>10kb) Multiome | 1072 | |
| chr12:103,956,327–103,957,900 | 8.5 kb | Proximal (<10kb) Multiome | 1090 | |
| chr12:103,965,409–103,966,472 | 85 bp | At TSS Multiome | 965 | |
| chr12:103,975,480–103,975,754 | 9.6 kb | Proximal (<10kb) | 74 | |
| chr12:104,049,717–104,050,283 | 84.2 kb | Distal (>10kb) Multiome | 295 | |
| chr12:104,063,714–104,065,209 | 98.7 kb | Distal (>10kb) Multiome | 950 | |
| chr12:104,137,119–104,139,117 | 172.4 kb | Distal (>10kb) Multiome | 796 | |
| chr12:104,154,657–104,155,425 | 189.2 kb | Distal (>10kb) Multiome | 127 | |
| chr12:104,215,473–104,216,381 | 250.0 kb | Distal (>10kb) Multiome | 514 | |
| chr12:105,107,164–105,108,312 | 1141.9 kb | Distal (>10kb) Multiome HiCAR | 1033 |
Genomic view of the TDG locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.