This gene encodes one of several TATA-binding protein (TBP)-associated factors (TAFs), which are integral subunits of the general transcription factor complex TFIID. TFIID recognizes the core promoter of many genes and nucleates the assembly of a transcription preinitiation complex containing RNA polymerase II and other initiation factors. The protein encoded by this gene contains an H4-like histone fold domain, and interacts with several subunits of TFIID including TBP and the histone-fold protein TAF10. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for TAF8. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TAF8 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TAF8, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr6:41,779,509–41,781,116 | 270.5 kb | Distal (>10kb) Multiome HiCAR | 857 | |
| chr6:41,786,100–41,787,779 | 264.0 kb | Distal (>10kb) Multiome HiCAR | 1106 | |
| chr6:41,894,347–41,896,221 | 155.1 kb | Distal (>10kb) Multiome | 822 | |
| chr6:41,920,834–41,921,901 | 129.3 kb | Distal (>10kb) Multiome | 955 | |
| chr6:41,939,116–41,939,782 | 111.1 kb | Distal (>10kb) Multiome | 401 | |
| chr6:41,940,689–41,942,238 | 108.7 kb | Distal (>10kb) Multiome | 798 | |
| chr6:41,965,433–41,966,075 | 84.8 kb | Distal (>10kb) Multiome | 152 | |
| chr6:42,030,637–42,031,169 | 19.5 kb | Distal (>10kb) Multiome | 297 | |
| chr6:42,044,107–42,044,485 | 6.0 kb | Proximal (<10kb) | 524 | |
| chr6:42,045,613–42,045,941 | 4.6 kb | Proximal (<10kb) | 448 | |
| chr6:42,048,292–42,049,051 | 1.8 kb | Proximal (<10kb) Multiome | 627 | |
| chr6:42,050,080–42,050,921 | 66 bp | At TSS Multiome | 912 | |
| chr6:42,104,179–42,104,979 | 53.9 kb | Distal (>10kb) Multiome | 486 | |
| chr6:42,136,520–42,137,140 | 86.3 kb | Distal (>10kb) Multiome | 555 | |
| chr6:42,142,113–42,142,966 | 92.0 kb | Distal (>10kb) Multiome | 489 | |
| chr6:42,166,489–42,167,048 | 116.2 kb | Distal (>10kb) Multiome | 451 | |
| chr6:42,177,902–42,178,547 | 127.7 kb | Distal (>10kb) Multiome | 161 | |
| chr6:42,260,672–42,261,201 | 210.5 kb | Distal (>10kb) Multiome | 301 |
Genomic view of the TAF8 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.