Many DNA-binding transcriptional activator proteins enhance the initiation rate of RNA polymerase II-mediated gene transcription by interacting functionally with the general transcription machinery bound at the basal promoter. Adaptor proteins are usually required for this activation, possibly to acetylate and destabilize nucleosomes, thereby relieving chromatin constraints at the promoter. The protein encoded by this gene is a transcriptional activator adaptor and has been found to be part of the PCAF histone acetylase complex. Several alternatively spliced transcript variants encoding different isoforms of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Oct 2009]
Transcription factors with Perturb-seq knockdown data for TADA2A. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TADA2A upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TADA2A, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr17:36,612,842–36,614,639 | 793.5 kb | Distal (>10kb) Multiome HiCAR | 111 | |
| chr17:37,124,503–37,125,029 | 282.2 kb | Distal (>10kb) Multiome | 41 | |
| chr17:37,358,363–37,360,066 | 47.6 kb | Distal (>10kb) Multiome | 859 | |
| chr17:37,406,418–37,407,500 | 69 bp | At TSS Multiome | 822 | |
| chr17:37,488,914–37,490,446 | 82.9 kb | Distal (>10kb) Multiome | 759 | |
| chr17:37,608,867–37,609,901 | 202.5 kb | Distal (>10kb) Multiome | 930 | |
| chr17:37,643,162–37,643,681 | 236.5 kb | Distal (>10kb) Multiome | 540 |
Genomic view of the TADA2A locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.