The polyamines putrescine, spermine, and spermidine are ubiquitous polycationic mediators of cell growth and differentiation. Spermidine synthase is one of four enzymes in the polyamine-biosynthetic pathway and carries out the final step of spermidine biosynthesis. This enzyme catalyzes the conversion of putrescine to spermidine using decarboxylated S-adenosylmethionine as the cofactor. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for SRM. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = SRM upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of SRM, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:10,793,659–10,795,336 | 266.0 kb | Distal (>10kb) Multiome | 450 | |
| chr1:10,796,172–10,796,997 | 263.3 kb | Distal (>10kb) Multiome | 574 | |
| chr1:10,835,650–10,836,437 | 224.0 kb | Distal (>10kb) Multiome | 422 | |
| chr1:10,866,886–10,867,599 | 192.8 kb | Distal (>10kb) Multiome | 364 | |
| chr1:11,011,823–11,013,364 | 47.6 kb | Distal (>10kb) Multiome | 974 | |
| chr1:11,045,518–11,045,887 | 9.9 kb | Proximal (<10kb) | 84 | |
| chr1:11,052,805–11,053,698 | 6.7 kb | Proximal (<10kb) Multiome | 314 | |
| chr1:11,059,469–11,060,508 | 90 bp | At TSS Multiome | 659 | |
| chr1:11,099,300–11,100,191 | 39.7 kb | Distal (>10kb) Multiome | 798 | |
| chr1:11,262,158–11,262,957 | 202.7 kb | Distal (>10kb) Multiome | 805 | |
| chr1:11,272,615–11,274,143 | 213.1 kb | Distal (>10kb) Multiome | 912 | |
| chr1:11,311,832–11,312,672 | 252.3 kb | Distal (>10kb) Multiome | 49 | |
| chr1:11,663,895–11,664,883 | 604.2 kb | Distal (>10kb) Multiome HiCAR | 644 |
Genomic view of the SRM locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.