The protein encoded by this gene belongs to the steroid 5-alpha reductase family, and polyprenol reductase subfamily. It is involved in the production of androgen 5-alpha-dihydrotestosterone (DHT) from testosterone, and maintenance of the androgen-androgen receptor activation pathway. This protein is also necessary for the conversion of polyprenol into dolichol, which is required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-linked glycosylation of proteins. Mutations in this gene are associated with congenital disorder of glycosylation type Iq. [provided by RefSeq, Mar 2011]
Transcription factors with Perturb-seq knockdown data for SRD5A3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = SRD5A3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of SRD5A3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr4:54,986,173–54,986,703 | 359.9 kb | Distal (>10kb) Multiome HiCAR | 116 | |
| chr4:55,124,658–55,126,127 | 220.7 kb | Distal (>10kb) Multiome | 263 | |
| chr4:55,169,439–55,169,958 | 176.5 kb | Distal (>10kb) Multiome | 88 | |
| chr4:55,256,726–55,257,189 | 89.2 kb | Distal (>10kb) Multiome | 74 | |
| chr4:55,280,261–55,281,766 | 64.8 kb | Distal (>10kb) Multiome | 162 | |
| chr4:55,346,084–55,347,178 | 152 bp | At TSS Multiome | 874 | |
| chr4:55,395,297–55,396,739 | 49.6 kb | Distal (>10kb) Multiome | 882 | |
| chr4:55,545,595–55,547,586 | 200.6 kb | Distal (>10kb) Multiome | 933 | |
| chr4:55,635,259–55,636,954 | 290.0 kb | Distal (>10kb) Multiome | 468 |
Genomic view of the SRD5A3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.