Spire proteins, such as SPIRE1, are highly conserved between species. They belong to the family of Wiskott-Aldrich homology region-2 (WH2) proteins, which are involved in actin organization (Kerkhoff et al., 2001 [PubMed 11747823]).[supplied by OMIM, Mar 2008]
Transcription factors with Perturb-seq knockdown data for SPIRE1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = SPIRE1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of SPIRE1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr18:12,376,065–12,378,406 | 280.6 kb | Distal (>10kb) Multiome HiCAR | 1012 | |
| chr18:12,407,513–12,408,521 | 250.2 kb | Distal (>10kb) Multiome HiCAR | 637 | |
| chr18:12,419,774–12,420,625 | 237.9 kb | Distal (>10kb) Multiome HiCAR | 579 | |
| chr18:12,420,869–12,421,324 | 237.0 kb | Distal (>10kb) Multiome | 417 | |
| chr18:12,657,070–12,658,817 | 17 bp | At TSS Multiome | 689 | |
| chr18:12,659,989–12,660,121 | 1.9 kb | Proximal (<10kb) | 148 | |
| chr18:12,702,219–12,703,287 | 44.7 kb | Distal (>10kb) Multiome | 869 | |
| chr18:12,766,645–12,767,371 | 109.1 kb | Distal (>10kb) Multiome | 392 | |
| chr18:12,776,564–12,778,096 | 118.9 kb | Distal (>10kb) Multiome | 123 | |
| chr18:12,862,247–12,862,768 | 204.5 kb | Distal (>10kb) Multiome | 116 | |
| chr18:12,883,233–12,884,627 | 226.1 kb | Distal (>10kb) Multiome | 904 | |
| chr18:12,946,945–12,948,745 | 289.8 kb | Distal (>10kb) Multiome | 1139 |
Genomic view of the SPIRE1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.