Transcription factors with Perturb-seq knockdown data for SPART-AS1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = SPART-AS1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of SPART-AS1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr13:36,130,286–36,131,909 | 215.2 kb | Distal (>10kb) Multiome | 348 | |
| chr13:36,297,356–36,298,329 | 48.5 kb | Distal (>10kb) Multiome | 597 | |
| chr13:36,298,442–36,299,112 | 47.5 kb | Distal (>10kb) Multiome | 192 | |
| chr13:36,324,257–36,325,131 | 21.8 kb | Distal (>10kb) Multiome | 143 | |
| chr13:36,337,249–36,337,726 | 8.7 kb | Proximal (<10kb) | 19 | |
| chr13:36,338,321–36,338,886 | 7.5 kb | Proximal (<10kb) | 14 | |
| chr13:36,345,510–36,347,162 | 289 bp | At TSS Multiome | 776 | |
| chr13:36,431,409–36,432,761 | 85.7 kb | Distal (>10kb) Multiome | 369 |
Genomic view of the SPART-AS1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.