This gene belongs to a family of Sp1 related genes that encode transcription factors that regulate transcription by binding to consensus GC- and GT-box regulatory elements in target genes. This protein contains a zinc finger DNA-binding domain and several transactivation domains, and has been reported to function as a bifunctional transcription factor that either stimulates or represses the transcription of numerous genes. Transcript variants encoding different isoforms have been described for this gene, and one has been reported to initiate translation from a non-AUG (AUA) start codon. Additional isoforms, resulting from the use of alternate downstream translation initiation sites, have also been noted. A related pseudogene has been identified on chromosome 13. [provided by RefSeq, Feb 2010]
Transcription factors with Perturb-seq knockdown data for SP3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = SP3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of SP3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr2:173,940,223–173,941,439 | 24.5 kb | Distal (>10kb) Multiome | 130 | |
| chr2:173,963,365–173,966,052 | 1.2 kb | Proximal (<10kb) Multiome | 1149 | |
| chr2:174,012,758–174,013,502 | 47.7 kb | Distal (>10kb) Multiome | 292 | |
| chr2:174,023,606–174,024,194 | 58.6 kb | Distal (>10kb) Multiome | 317 | |
| chr2:174,039,708–174,040,389 | 74.7 kb | Distal (>10kb) Multiome | 694 | |
| chr2:174,053,124–174,054,219 | 88.2 kb | Distal (>10kb) Multiome | 132 | |
| chr2:174,247,891–174,249,190 | 283.3 kb | Distal (>10kb) Multiome | 1036 |
Genomic view of the SP3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.