Transcription factors with Perturb-seq knockdown data for SMANTIS. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = SMANTIS upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of SMANTIS, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr2:69,642,875–69,644,362 | 155.2 kb | Distal (>10kb) Multiome | 960 | |
| chr2:69,741,315–69,742,479 | 57.0 kb | Distal (>10kb) Multiome | 894 | |
| chr2:69,798,793–69,799,774 | 239 bp | At TSS Multiome | 417 | |
| chr2:69,829,001–69,830,537 | 30.5 kb | Distal (>10kb) Multiome | 1005 | |
| chr2:69,914,102–69,915,874 | 115.8 kb | Distal (>10kb) Multiome | 1112 | |
| chr2:70,084,998–70,085,484 | 286.2 kb | Distal (>10kb) Multiome | 345 | |
| chr2:70,086,194–70,088,572 | 288.0 kb | Distal (>10kb) Multiome | 1204 | |
| chr2:70,089,216–70,090,447 | 290.8 kb | Distal (>10kb) Multiome | 459 |
Genomic view of the SMANTIS locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.