SMAD9
SMAD family member 9 | SMAD8, SMAD8/9, MADH6, MADH9

The protein encoded by this gene is a member of the SMAD family, which transduces signals from TGF-beta family members. The encoded protein is activated by bone morphogenetic proteins and interacts with SMAD4. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2010]

Member of: DE-3 DE-3.16 Developmental clusters: GC6
Biological processes 40 terms
BMP signaling pathway (GO:0030509)BMP signaling pathway (GO:0030509)BMP signaling pathway (GO:0030509)BMP signaling pathway (GO:0030509)DNA-binding transcription factor activity (GO:0003700)DNA-binding transcription factor activity, RNA polymerase II-specific (GO:0000981)DNA-binding transcription factor activity, RNA polymerase II-specific (GO:0000981)I-SMAD binding (GO:0070411)RNA polymerase II cis-regulatory region sequence-specific DNA binding (GO:0000978)RNA polymerase II cis-regulatory region sequence-specific DNA binding (GO:0000978)SMAD protein complex (GO:0071141)SMAD protein signal transduction (GO:0060395)SMAD protein signal transduction (GO:0060395)SMAD protein signal transduction (GO:0060395)anatomical structure morphogenesis (GO:0009653)cell differentiation (GO:0030154)cellular response to BMP stimulus (GO:0071773)cellular response to growth factor stimulus (GO:0071363)chromatin (GO:0000785)cytoplasm (GO:0005737)cytoplasm (GO:0005737)cytosol (GO:0005829)cytosol (GO:0005829)developmental process (GO:0032502)heteromeric SMAD protein complex (GO:0071144)intracellular iron ion homeostasis (GO:0006879)nucleoplasm (GO:0005654)nucleoplasm (GO:0005654)nucleus (GO:0005634)nucleus (GO:0005634)nucleus (GO:0005634)osteoblast differentiation (GO:0001649)positive regulation of transcription by RNA polymerase II (GO:0045944)protein binding (GO:0005515)regulation of DNA-templated transcription (GO:0006355)regulation of transcription by RNA polymerase II (GO:0006357)stem cell differentiation (GO:0048863)transcription regulator complex (GO:0005667)transforming growth factor beta receptor signaling pathway (GO:0007179)transforming growth factor beta receptor superfamily signaling pathway (GO:0141091)
Expression (TPM)
SMAD9 — as a Regulated Gene

TFs regulating SMAD9 0 TFs

Transcription factors with Perturb-seq knockdown data for SMAD9. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = SMAD9 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

Data: Effect:
TF Mean coef Binding Outlier TF→Gene link

Elements linked to SMAD9

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of SMAD9, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Accessibility Element Dist. to TSS Link type TFs
chr13:36,673,749–36,675,136 246.9 kb Distal (>10kb) Multiome 286
chr13:36,761,910–36,762,570 158.7 kb Distal (>10kb) Multiome 247
chr13:36,818,153–36,820,403 101.7 kb Distal (>10kb) Multiome 690
chr13:36,919,257–36,920,884 751 bp At TSS Multiome 481
chr13:36,998,372–37,001,433 78.4 kb Distal (>10kb) Multiome 1090
chr13:37,058,947–37,060,264 138.8 kb Distal (>10kb) Multiome 801

Genome Browser

Genomic view of the SMAD9 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.

chr13:36,663,749 – 37,070,264
Proximal 1 kb Distal 10 kb Multiome HiCAR ATAC-seq RNA-seq