Transcription factors with Perturb-seq knockdown data for SMAD3-DT. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = SMAD3-DT upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of SMAD3-DT, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr15:67,063,703–67,064,666 | 601 bp | At TSS | 769 | |
| chr15:67,064,798–67,066,190 | at TSS | At TSS | 881 | |
| chr15:67,067,506–67,068,588 | 2.2 kb | Proximal (<10kb) | 341 | |
| chr15:67,070,163–67,070,402 | 4.9 kb | Proximal (<10kb) | 48 | |
| chr15:67,071,011–67,071,208 | 5.7 kb | Proximal (<10kb) | 30 |
Genomic view of the SMAD3-DT locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.