The reversible posttranslational modification of proteins by the addition of small ubiquitin-like SUMO proteins (see SUMO1; MIM 601912) is required for numerous biologic processes. SUMO-specific proteases, such as SENP5, are responsible for the initial processing of SUMO precursors to generate a C-terminal diglycine motif required for the conjugation reaction. They also have isopeptidase activity for the removal of SUMO from high molecular mass SUMO conjugates (Di Bacco et al., 2006 [PubMed 16738315]).[supplied by OMIM, Jun 2009]
Transcription factors with Perturb-seq knockdown data for SENP5. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = SENP5 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of SENP5, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr3:196,568,218–196,569,450 | 299.2 kb | Distal (>10kb) Multiome | 853 | |
| chr3:196,631,880–196,633,250 | 235.3 kb | Distal (>10kb) Multiome | 879 | |
| chr3:196,639,198–196,640,059 | 228.2 kb | Distal (>10kb) Multiome | 438 | |
| chr3:196,665,925–196,666,686 | 201.6 kb | Distal (>10kb) Multiome | 439 | |
| chr3:196,711,809–196,713,001 | 155.5 kb | Distal (>10kb) Multiome | 782 | |
| chr3:196,739,555–196,740,517 | 128.0 kb | Distal (>10kb) Multiome | 736 | |
| chr3:196,867,462–196,868,618 | 57 bp | At TSS Multiome | 794 | |
| chr3:196,941,814–196,943,294 | 74.6 kb | Distal (>10kb) Multiome | 1027 | |
| chr3:196,968,365–196,969,147 | 100.8 kb | Distal (>10kb) Multiome | 597 | |
| chr3:196,969,208–196,970,172 | 102.1 kb | Distal (>10kb) Multiome | 714 | |
| chr3:197,002,398–197,004,003 | 135.2 kb | Distal (>10kb) Multiome | 569 | |
| chr3:197,028,743–197,030,204 | 161.9 kb | Distal (>10kb) Multiome | 646 |
Genomic view of the SENP5 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.