SEMA4D
semaphorin 4D | CD100, FLJ39737, coll-4, C9orf164, SEMAJ

Enables identical protein binding activity; semaphorin receptor binding activity; and transmembrane signaling receptor activity. Involved in several processes, including positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction; regulation of neuron projection development; and regulation of primary metabolic process. Located in microtubule organizing center; nucleoplasm; and plasma membrane. [provided by Alliance of Genome Resources, Jul 2025]

Member of: DE-3 DE-3.10
Biological processes 67 terms
GABA-ergic synapse (GO:0098982)axon guidance (GO:0007411)bone trabecula morphogenesis (GO:0061430)bone trabecula morphogenesis (GO:0061430)cell adhesion (GO:0007155)chemorepellent activity (GO:0045499)extracellular region (GO:0005576)extracellular region (GO:0005576)extracellular region (GO:0005576)extracellular region (GO:0005576)identical protein binding (GO:0042802)immune response (GO:0006955)leukocyte aggregation (GO:0070486)membrane (GO:0016020)negative chemotaxis (GO:0050919)negative regulation of apoptotic process (GO:0043066)negative regulation of cell adhesion (GO:0007162)negative regulation of osteoblast differentiation (GO:0045668)negative regulation of osteoblast differentiation (GO:0045668)negative regulation of transcription by RNA polymerase II (GO:0000122)negative regulation of transcription by RNA polymerase II (GO:0000122)negative regulation of transcription by RNA polymerase II (GO:0000122)neural crest cell migration (GO:0001755)neuropilin binding (GO:0038191)ossification involved in bone maturation (GO:0043931)ossification involved in bone maturation (GO:0043931)plasma membrane (GO:0005886)plasma membrane (GO:0005886)plasma membrane (GO:0005886)plasma membrane (GO:0005886)positive regulation of GTPase activity (GO:0043547)positive regulation of Rho protein signal transduction (GO:0035025)positive regulation of Rho protein signal transduction (GO:0035025)positive regulation of axonogenesis (GO:0050772)positive regulation of cell migration (GO:0030335)positive regulation of cell migration (GO:0030335)positive regulation of cell migration (GO:0030335)positive regulation of cell migration (GO:0030335)positive regulation of collateral sprouting (GO:0048672)positive regulation of inhibitory synapse assembly (GO:1905704)positive regulation of inhibitory synapse assembly (GO:1905704)positive regulation of intracellular signal transduction (GO:1902533)positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction (GO:0051897)positive regulation of protein phosphorylation (GO:0001934)postsynaptic membrane (GO:0045211)protein binding (GO:0005515)receptor ligand activity (GO:0048018)receptor ligand activity (GO:0048018)regulation of cell projection organization (GO:0031344)regulation of cell shape (GO:0008360)regulation of dendrite morphogenesis (GO:0048814)regulation of synapse assembly (GO:0051963)semaphorin receptor binding (GO:0030215)semaphorin receptor binding (GO:0030215)semaphorin receptor binding (GO:0030215)semaphorin receptor binding (GO:0030215)semaphorin receptor complex (GO:0002116)semaphorin receptor complex (GO:0002116)semaphorin-plexin signaling pathway (GO:0071526)semaphorin-plexin signaling pathway (GO:0071526)semaphorin-plexin signaling pathway (GO:0071526)semaphorin-plexin signaling pathway (GO:0071526)semaphorin-plexin signaling pathway (GO:0071526)signaling receptor activity (GO:0038023)signaling receptor binding (GO:0005102)signaling receptor binding (GO:0005102)transmembrane signaling receptor activity (GO:0004888)
Expression (TPM)
SEMA4D — as a Regulated Gene

TFs regulating SEMA4D 0 TFs

Transcription factors with Perturb-seq knockdown data for SEMA4D. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = SEMA4D upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

Data: Effect:
TF Mean coef Binding Outlier TF→Gene link

Elements linked to SEMA4D

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of SEMA4D, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Accessibility Element Dist. to TSS Link type TFs
chr9:89,225,983–89,226,479 271.8 kb Distal (>10kb) Multiome 72
chr9:89,295,298–89,295,875 202.5 kb Distal (>10kb) Multiome 98
chr9:89,310,238–89,311,866 187.2 kb Distal (>10kb) Multiome 945
chr9:89,317,972–89,319,093 179.5 kb Distal (>10kb) Multiome 1083
chr9:89,435,291–89,435,862 62.6 kb Distal (>10kb) Multiome HiCAR 216
chr9:89,497,231–89,498,509 64 bp At TSS Multiome 374
chr9:89,603,012–89,604,575 105.0 kb Distal (>10kb) Multiome 537
chr9:89,604,751–89,607,232 108.7 kb Distal (>10kb) Multiome 689
chr9:89,661,587–89,662,555 164.0 kb Distal (>10kb) Multiome 313
chr9:89,675,046–89,675,613 177.1 kb Distal (>10kb) Multiome 98
chr9:89,677,983–89,678,537 180.1 kb Distal (>10kb) Multiome 119
chr9:89,705,274–89,706,387 207.6 kb Distal (>10kb) Multiome 54

Genome Browser

Genomic view of the SEMA4D locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.

chr9:89,215,983 – 89,716,387
Proximal 1 kb Distal 10 kb Multiome HiCAR ATAC-seq RNA-seq