Transcription factors with Perturb-seq knockdown data for RTL8A. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = RTL8A upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of RTL8A, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chrX:134,796,182–134,797,389 | 255.0 kb | Distal (>10kb) Multiome | 527 | |
| chrX:134,802,923–134,803,464 | 248.8 kb | Distal (>10kb) Multiome | 62 | |
| chrX:134,807,010–134,807,486 | 244.9 kb | Distal (>10kb) Multiome | 401 | |
| chrX:134,914,755–134,915,732 | 136.8 kb | Distal (>10kb) Multiome | 575 | |
| chrX:135,003,873–135,004,623 | 47.9 kb | Distal (>10kb) Multiome | 369 | |
| chrX:135,022,346–135,022,840 | 29.6 kb | Distal (>10kb) Multiome | 290 | |
| chrX:135,031,918–135,032,932 | 19.9 kb | Distal (>10kb) Multiome | 428 | |
| chrX:135,051,315–135,052,574 | 50 bp | At TSS Multiome | 523 | |
| chrX:135,097,570–135,099,197 | 46.7 kb | Distal (>10kb) Multiome | 354 | |
| chrX:135,343,574–135,345,233 | 292.6 kb | Distal (>10kb) Multiome | 657 | |
| chrX:136,880,062–136,881,160 | 1828.7 kb | Distal (>10kb) Multiome HiCAR | 642 |
Genomic view of the RTL8A locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.