Cytoplasmic ribosomes, organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes ribosomal protein S4, a component of the 40S subunit. Ribosomal protein S4 is the only ribosomal protein known to be encoded by more than one gene, namely this gene and ribosomal protein S4, Y-linked (RPS4Y). The 2 isoforms encoded by these genes are not identical, but are functionally equivalent. Ribosomal protein S4 belongs to the S4E family of ribosomal proteins. This gene is not subject to X-inactivation. It has been suggested that haploinsufficiency of the ribosomal protein S4 genes plays a role in Turner syndrome; however, this hypothesis is controversial. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for RPS4X. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = RPS4X upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of RPS4X, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chrX:72,018,244–72,018,883 | 258.6 kb | Distal (>10kb) Multiome | 577 | |
| chrX:72,115,329–72,115,816 | 161.7 kb | Distal (>10kb) Multiome | 75 | |
| chrX:72,131,595–72,132,294 | 145.2 kb | Distal (>10kb) Multiome | 266 | |
| chrX:72,181,219–72,182,040 | 95.7 kb | Distal (>10kb) Multiome | 619 | |
| chrX:72,238,686–72,239,464 | 38.2 kb | Distal (>10kb) Multiome | 448 | |
| chrX:72,276,823–72,277,642 | 10 bp | At TSS Multiome | 768 | |
| chrX:72,554,193–72,555,226 | 277.5 kb | Distal (>10kb) Multiome | 207 | |
| chrX:72,572,355–72,573,221 | 295.7 kb | Distal (>10kb) Multiome | 541 |
Genomic view of the RPS4X locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.