Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi. Multiple alternatively spliced transcript variants that encode the same proteins have been identified.[provided by RefSeq, Sep 2008]
Transcription factors with Perturb-seq knockdown data for RPS27A. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = RPS27A upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of RPS27A, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr2:54,945,613–54,946,419 | 286.6 kb | Distal (>10kb) Multiome | 135 | |
| chr2:55,048,854–55,051,460 | 182.0 kb | Distal (>10kb) Multiome | 1172 | |
| chr2:55,133,866–55,134,461 | 98.6 kb | Distal (>10kb) Multiome | 417 | |
| chr2:55,223,149–55,223,979 | 9.1 kb | Proximal (<10kb) Multiome | 386 | |
| chr2:55,231,636–55,233,143 | 289 bp | At TSS Multiome | 993 | |
| chr2:55,268,856–55,269,481 | 36.6 kb | Distal (>10kb) Multiome | 1083 | |
| chr2:55,279,327–55,280,579 | 47.1 kb | Distal (>10kb) Multiome | 219 | |
| chr2:55,281,727–55,282,873 | 49.6 kb | Distal (>10kb) Multiome | 943 | |
| chr2:55,418,822–55,420,474 | 187.3 kb | Distal (>10kb) Multiome | 1181 | |
| chr2:55,518,709–55,520,527 | 286.9 kb | Distal (>10kb) Multiome | 882 |
Genomic view of the RPS27A locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.