Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L14E family of ribosomal proteins. It contains a basic region-leucine zipper (bZIP)-like domain. The protein is located in the cytoplasm. This gene contains a trinucleotide (GCT) repeat tract whose length is highly polymorphic; these triplet repeats result in a stretch of alanine residues in the encoded protein. Transcript variants utilizing alternative polyA signals and alternative 5'-terminal exons exist but all encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for RPL14. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = RPL14 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of RPL14, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr3:40,291,090–40,292,047 | 165.7 kb | Distal (>10kb) Multiome | 87 | |
| chr3:40,308,903–40,310,435 | 147.7 kb | Distal (>10kb) Multiome | 940 | |
| chr3:40,386,652–40,387,704 | 70.2 kb | Distal (>10kb) Multiome | 280 | |
| chr3:40,452,726–40,453,738 | 4.1 kb | Proximal (<10kb) Multiome | 787 | |
| chr3:40,456,939–40,457,754 | 6 bp | At TSS Multiome | 794 | |
| chr3:40,476,853–40,477,451 | 19.8 kb | Distal (>10kb) Multiome | 534 | |
| chr3:40,505,545–40,506,480 | 48.7 kb | Distal (>10kb) Multiome | 700 | |
| chr3:40,524,355–40,525,405 | 67.5 kb | Distal (>10kb) Multiome | 904 | |
| chr3:40,615,244–40,616,130 | 158.4 kb | Distal (>10kb) Multiome | 206 | |
| chr3:41,961,164–41,962,778 | 1504.9 kb | Distal (>10kb) Multiome HiCAR | 829 |
Genomic view of the RPL14 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.