ROCK2
Rho associated coiled-coil containing protein kinase 2

The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq, Jul 2008]

Member of: DE-2 DE-2.1
Biological processes 102 terms
ATP binding (GO:0005524)RNA binding (GO:0003723)Rho protein signal transduction (GO:0007266)Rho protein signal transduction (GO:0007266)Rho protein signal transduction (GO:0007266)Rho protein signal transduction (GO:0007266)Rho-dependent protein serine/threonine kinase activity (GO:0072518)Rho-dependent protein serine/threonine kinase activity (GO:0072518)Rho-dependent protein serine/threonine kinase activity (GO:0072518)Rho-dependent protein serine/threonine kinase activity (GO:0072518)actin cytoskeleton organization (GO:0030036)actin cytoskeleton organization (GO:0030036)actomyosin structure organization (GO:0031032)aortic valve morphogenesis (GO:0003180)blood vessel diameter maintenance (GO:0097746)cellular response to acetylcholine (GO:1905145)centrosome (GO:0005813)centrosome (GO:0005813)centrosome (GO:0005813)centrosome duplication (GO:0051298)cortical actin cytoskeleton organization (GO:0030866)cytoplasm (GO:0005737)cytoplasm (GO:0005737)cytoplasm (GO:0005737)cytoplasmic ribonucleoprotein granule (GO:0036464)cytoskeleton (GO:0005856)cytosol (GO:0005829)cytosol (GO:0005829)embryonic morphogenesis (GO:0048598)endopeptidase activator activity (GO:0061133)epithelial to mesenchymal transition (GO:0001837)host-mediated perturbation of viral process (GO:0044788)mRNA destabilization (GO:0061157)mitotic cytokinesis (GO:0000281)negative regulation of angiogenesis (GO:0016525)negative regulation of angiogenesis (GO:0016525)negative regulation of bicellular tight junction assembly (GO:1903347)negative regulation of bicellular tight junction assembly (GO:1903347)negative regulation of biomineral tissue development (GO:0070168)negative regulation of gene expression (GO:0010629)negative regulation of nitric oxide biosynthetic process (GO:0045019)negative regulation of nitric oxide biosynthetic process (GO:0045019)negative regulation of protein localization to lysosome (GO:0150033)nucleus (GO:0005634)plasma membrane (GO:0005886)positive regulation of MAPK cascade (GO:0043410)positive regulation of amyloid precursor protein catabolic process (GO:1902993)positive regulation of amyloid-beta formation (GO:1902004)positive regulation of cardiac muscle hypertrophy (GO:0010613)positive regulation of cell migration (GO:0030335)positive regulation of centrosome duplication (GO:0010825)positive regulation of connective tissue growth factor production (GO:0032723)positive regulation of connective tissue replacement (GO:1905205)positive regulation of fibroblast growth factor production (GO:0090271)positive regulation of gene expression (GO:0010628)positive regulation of protein localization to early endosome (GO:1902966)positive regulation of stress fiber assembly (GO:0051496)positive regulation of stress fiber assembly (GO:0051496)protease binding (GO:0002020)protein binding (GO:0005515)protein kinase activity (GO:0004672)protein localization to plasma membrane (GO:0072659)protein localization to plasma membrane (GO:0072659)protein localization to plasma membrane (GO:0072659)protein phosphorylation (GO:0006468)protein serine kinase activity (GO:0106310)protein serine/threonine kinase activity (GO:0004674)protein serine/threonine kinase activity (GO:0004674)protein serine/threonine kinase activity (GO:0004674)protein serine/threonine kinase activity (GO:0004674)regulation of actin cytoskeleton organization (GO:0032956)regulation of actin cytoskeleton organization (GO:0032956)regulation of actin cytoskeleton organization (GO:0032956)regulation of amyloid-beta formation (GO:1902003)regulation of angiotensin-activated signaling pathway (GO:0110061)regulation of cell adhesion (GO:0030155)regulation of cell junction assembly (GO:1901888)regulation of cell motility (GO:2000145)regulation of cellular response to hypoxia (GO:1900037)regulation of circadian rhythm (GO:0042752)regulation of establishment of cell polarity (GO:2000114)regulation of establishment of cell polarity (GO:2000114)regulation of establishment of endothelial barrier (GO:1903140)regulation of establishment of endothelial barrier (GO:1903140)regulation of establishment of endothelial barrier (GO:1903140)regulation of focal adhesion assembly (GO:0051893)regulation of focal adhesion assembly (GO:0051893)regulation of keratinocyte differentiation (GO:0045616)regulation of keratinocyte differentiation (GO:0045616)regulation of nervous system process (GO:0031644)regulation of stress fiber assembly (GO:0051492)regulation of stress fiber assembly (GO:0051492)response to angiotensin (GO:1990776)response to ischemia (GO:0002931)response to transforming growth factor beta (GO:0071559)signal transduction (GO:0007165)small GTPase binding (GO:0031267)smooth muscle contraction (GO:0006939)smooth muscle contraction (GO:0006939)structural molecule activity (GO:0005198)tau protein binding (GO:0048156)tau-protein kinase activity (GO:0050321)
Expression (TPM)
ROCK2 — as a Regulated Gene

TFs regulating ROCK2 0 TFs

Transcription factors with Perturb-seq knockdown data for ROCK2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ROCK2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

Data: Effect:
TF Mean coef Binding Outlier TF→Gene link

Elements linked to ROCK2

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ROCK2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Accessibility Element Dist. to TSS Link type TFs
chr2:11,129,508–11,130,490 214.6 kb Distal (>10kb) Multiome HiCAR 660
chr2:11,132,494–11,133,872 211.7 kb Distal (>10kb) Multiome HiCAR 774
chr2:11,154,551–11,156,096 189.3 kb Distal (>10kb) Multiome 845
chr2:11,343,518–11,345,475 113 bp At TSS Multiome 1010
chr2:11,465,452–11,466,561 121.5 kb Distal (>10kb) Multiome 1149
chr2:11,481,838–11,483,838 138.2 kb Distal (>10kb) Multiome 777
chr2:11,539,056–11,540,222 194.8 kb Distal (>10kb) Multiome 709
chr2:11,550,790–11,551,875 206.8 kb Distal (>10kb) Multiome 171
chr2:11,559,501–11,560,048 215.2 kb Distal (>10kb) Multiome 146
chr2:11,582,389–11,583,691 238.6 kb Distal (>10kb) Multiome 543

Genome Browser

Genomic view of the ROCK2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.

chr2:11,119,508 – 11,593,691
Proximal 1 kb Distal 10 kb Multiome HiCAR ATAC-seq RNA-seq
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