The protein encoded by this gene contains a RING finger motif and an FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating enzymes (E2), including UBE2E1/UBCH6, UBE2E2, and UBE2E3, and may act as an ubiquitin ligase (E3) in the ubiquitination of certain nuclear proteins. This protein is also known to play a role in the DNA damage response and depletion of this protein causes cell growth inhibition and cell cycle arrest. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]
Transcription factors with Perturb-seq knockdown data for RNF8. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = RNF8 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of RNF8, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr6:37,059,338–37,060,100 | 294.3 kb | Distal (>10kb) Multiome | 824 | |
| chr6:37,071,640–37,072,385 | 282.1 kb | Distal (>10kb) Multiome | 281 | |
| chr6:37,102,284–37,103,074 | 251.3 kb | Distal (>10kb) Multiome | 855 | |
| chr6:37,125,557–37,126,783 | 227.9 kb | Distal (>10kb) Multiome | 528 | |
| chr6:37,137,028–37,138,409 | 216.2 kb | Distal (>10kb) Multiome | 735 | |
| chr6:37,141,368–37,141,988 | 212.3 kb | Distal (>10kb) Multiome | 239 | |
| chr6:37,168,853–37,171,673 | 184.0 kb | Distal (>10kb) Multiome | 1096 | |
| chr6:37,172,048–37,172,496 | 181.6 kb | Distal (>10kb) Multiome | 818 | |
| chr6:37,221,712–37,222,953 | 131.3 kb | Distal (>10kb) Multiome | 747 | |
| chr6:37,257,338–37,258,482 | 96.2 kb | Distal (>10kb) Multiome | 890 | |
| chr6:37,319,150–37,319,973 | 34.4 kb | Distal (>10kb) Multiome | 54 | |
| chr6:37,353,783–37,354,458 | 25 bp | At TSS Multiome | 794 | |
| chr6:37,432,813–37,433,740 | 79.3 kb | Distal (>10kb) Multiome | 901 | |
| chr6:37,484,079–37,484,645 | 130.3 kb | Distal (>10kb) Multiome | 222 | |
| chr6:37,499,465–37,500,524 | 146.0 kb | Distal (>10kb) Multiome | 714 | |
| chr6:37,565,348–37,566,769 | 211.9 kb | Distal (>10kb) Multiome | 518 | |
| chr6:37,647,989–37,649,458 | 294.8 kb | Distal (>10kb) Multiome | 333 |
Genomic view of the RNF8 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.