The protein encoded by this gene is a RING-type E3 ubiquitin ligase and is predicted to contain a transmembrane domain, a protease-associated domain, an ectodomain, and a cytoplasmic RING domain. This protein is thought to negatively regulate Wnt signaling, and expression of this gene results in an increase in ubiquitination of frizzled receptors, an alteration in their subcellular distribution, resulting in reduced surface levels of these receptors. Mutations in this gene have been reported in multiple tumor cells, including colorectal and endometrial cancers. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2015]
Transcription factors with Perturb-seq knockdown data for RNF43. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = RNF43 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of RNF43, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr17:58,156,945–58,157,756 | 260.4 kb | Distal (>10kb) Multiome | 235 | |
| chr17:58,218,800–58,219,654 | 198.3 kb | Distal (>10kb) Multiome | 890 | |
| chr17:58,249,147–58,250,121 | 167.9 kb | Distal (>10kb) Multiome | 737 | |
| chr17:58,324,153–58,325,169 | 93.0 kb | Distal (>10kb) Multiome | 274 | |
| chr17:58,338,012–58,338,430 | 79.4 kb | Distal (>10kb) Multiome HiCAR | 559 | |
| chr17:58,339,009–58,339,515 | 78.3 kb | Distal (>10kb) Multiome | 382 | |
| chr17:58,351,751–58,352,762 | 65.3 kb | Distal (>10kb) Multiome | 990 | |
| chr17:58,417,103–58,417,223 | 371 bp | At TSS | 138 | |
| chr17:58,417,453–58,417,876 | at TSS | At TSS | 859 | |
| chr17:58,487,466–58,488,518 | 70.2 kb | Distal (>10kb) Multiome | 222 | |
| chr17:58,513,911–58,515,157 | 97.0 kb | Distal (>10kb) Multiome | 585 | |
| chr17:58,517,239–58,519,377 | 100.4 kb | Distal (>10kb) Multiome | 1140 | |
| chr17:58,527,884–58,528,411 | 110.6 kb | Distal (>10kb) Multiome | 79 | |
| chr17:58,531,392–58,532,430 | 114.4 kb | Distal (>10kb) Multiome | 323 | |
| chr17:58,630,482–58,632,252 | 214.1 kb | Distal (>10kb) Multiome | 1257 | |
| chr17:58,657,864–58,659,830 | 241.6 kb | Distal (>10kb) Multiome | 1269 | |
| chr17:58,669,900–58,670,548 | 252.7 kb | Distal (>10kb) Multiome | 52 | |
| chr17:58,678,911–58,679,743 | 261.9 kb | Distal (>10kb) Multiome | 889 | |
| chr17:58,692,284–58,692,956 | 275.0 kb | Distal (>10kb) Multiome | 1038 |
Genomic view of the RNF43 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.