The protein encoded by this gene shares similarity with BRE1 of S. cerevisiae. The protein encoded by this human gene is an E3 ubiquitin ligase that regulates chromosome structure by monoubiquitinating histone H2B. This protein acts as a putative tumor suppressor and positively regulates the p53 tumor suppressor as well as numerous histone H2A and H2B genes. In contrast, this protein also suppresses the expression of several protooncogenes and growth-related genes, including many genes that are induced by epidermal growth factor. This gene selectively suppresses the expression of some genes by interfering with chromatin recruitment of transcription elongation factor SII (TFIIS). [provided by RefSeq, Feb 2012]
Transcription factors with Perturb-seq knockdown data for RNF20. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = RNF20 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of RNF20, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr9:101,397,915–101,399,348 | 135.1 kb | Distal (>10kb) Multiome | 861 | |
| chr9:101,449,087–101,449,796 | 84.5 kb | Distal (>10kb) Multiome | 494 | |
| chr9:101,458,960–101,459,540 | 74.7 kb | Distal (>10kb) Multiome HiCAR | 253 | |
| chr9:101,460,651–101,461,628 | 72.7 kb | Distal (>10kb) Multiome | 103 | |
| chr9:101,485,920–101,487,719 | 46.8 kb | Distal (>10kb) Multiome | 692 | |
| chr9:101,530,178–101,530,399 | 3.5 kb | Proximal (<10kb) | 173 | |
| chr9:101,533,168–101,534,447 | 111 bp | At TSS Multiome | 723 | |
| chr9:101,737,499–101,739,011 | 204.4 kb | Distal (>10kb) Multiome | 280 |
Genomic view of the RNF20 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.