This gene encodes a member of the Rho family of GTPases. This protein can activate PAK1 and JNK1, and can induce filopodium formation and stress fiber dissolution. It may also mediate the effects of WNT1 signaling in the regulation of cell morphology, cytoskeletal organization, and cell proliferation. A non-coding transcript variant of this gene results from naturally occurring read-through transcription between this locus and the neighboring DUSP5P (dual specificity phosphatase 5 pseudogene) locus.[provided by RefSeq, Mar 2011]
Transcription factors with Perturb-seq knockdown data for RHOU. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = RHOU upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of RHOU, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:228,457,070–228,458,723 | 277.6 kb | Distal (>10kb) Multiome | 800 | |
| chr1:228,458,870–228,459,670 | 276.1 kb | Distal (>10kb) Multiome | 503 | |
| chr1:228,464,228–228,465,055 | 270.7 kb | Distal (>10kb) Multiome | 204 | |
| chr1:228,486,589–228,487,963 | 248.2 kb | Distal (>10kb) Multiome | 1041 | |
| chr1:228,647,259–228,648,459 | 87.4 kb | Distal (>10kb) Multiome | 684 | |
| chr1:228,735,011–228,736,739 | 59 bp | At TSS Multiome | 664 | |
| chr1:228,781,279–228,781,746 | 46.0 kb | Distal (>10kb) Multiome | 238 | |
| chr1:228,809,633–228,810,556 | 74.7 kb | Distal (>10kb) Multiome | 201 | |
| chr1:228,848,989–228,849,550 | 113.8 kb | Distal (>10kb) Multiome | 204 | |
| chr1:229,027,699–229,028,244 | 292.6 kb | Distal (>10kb) Multiome | 280 | |
| chr1:229,090,571–229,091,190 | 355.3 kb | Distal (>10kb) Multiome HiCAR | 31 | |
| chr1:229,093,644–229,094,437 | 358.6 kb | Distal (>10kb) Multiome HiCAR | 253 |
Genomic view of the RHOU locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.