This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The encoded protein is an important signalling protein for sarcomere assembly and has been shown to play a significant role in the exocytosis of the solute carrier family 2, facilitated glucose transporter member 4 and other proteins, possibly acting as the signal that turns on the membrane fusion machinery. Three related pseudogene have been identified on chromosomes 2 and 14. [provided by RefSeq, Aug 2011]
Transcription factors with Perturb-seq knockdown data for RHOQ. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = RHOQ upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of RHOQ, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr2:46,296,415–46,298,276 | 245.5 kb | Distal (>10kb) Multiome | 911 | |
| chr2:46,499,830–46,500,537 | 42.5 kb | Distal (>10kb) Multiome | 657 | |
| chr2:46,541,624–46,542,151 | 772 bp | At TSS Multiome | 610 | |
| chr2:46,542,212–46,544,274 | 214 bp | At TSS Multiome | 785 | |
| chr2:46,551,009–46,551,446 | 8.3 kb | Proximal (<10kb) | 51 | |
| chr2:46,616,071–46,617,512 | 74.3 kb | Distal (>10kb) Multiome | 955 | |
| chr2:46,698,279–46,700,093 | 156.3 kb | Distal (>10kb) Multiome | 974 | |
| chr2:46,704,551–46,705,027 | 162.1 kb | Distal (>10kb) Multiome | 109 |
Genomic view of the RHOQ locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.