Transcription factors with Perturb-seq knockdown data for RFLNB. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = RFLNB upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of RFLNB, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr17:180,354–181,916 | 264.3 kb | Distal (>10kb) Multiome | 167 | |
| chr17:385,385–386,511 | 59.7 kb | Distal (>10kb) Multiome | 511 | |
| chr17:409,399–411,454 | 35.7 kb | Distal (>10kb) Multiome | 665 | |
| chr17:714,554–715,298 | 268.9 kb | Distal (>10kb) Multiome | 798 | |
| chr17:731,870–733,144 | 286.5 kb | Distal (>10kb) Multiome | 602 |
Genomic view of the RFLNB locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.