This gene encodes the smallest subunit of the replication factor C complex, which consists of five distinct subunits (140, 40, 38, 37, and 36 kDa) and is required for DNA replication. This subunit interacts with the C-terminal region of proliferating cell nuclear antigen and is required to open and load proliferating cell nuclear antigen onto DNA during S phase. It is a member of the AAA+ (ATPases associated with various cellular activities) ATPase family and forms a core complex with the 38 and 40 kDa subunits that possesses DNA-dependent ATPase activity. A related pseudogene has been identified on chromosome 9. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2016]
Transcription factors with Perturb-seq knockdown data for RFC5. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = RFC5 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of RFC5, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:117,968,587–117,969,787 | 47.5 kb | Distal (>10kb) Multiome | 508 | |
| chr12:118,016,461–118,017,217 | 40 bp | At TSS Multiome | 782 | |
| chr12:118,059,537–118,060,430 | 43.1 kb | Distal (>10kb) Multiome | 222 | |
| chr12:118,060,617–118,061,718 | 44.6 kb | Distal (>10kb) Multiome | 532 | |
| chr12:118,103,306–118,104,597 | 87.2 kb | Distal (>10kb) Multiome | 730 | |
| chr12:118,119,571–118,120,301 | 103.0 kb | Distal (>10kb) Multiome | 62 | |
| chr12:118,120,669–118,121,341 | 104.2 kb | Distal (>10kb) Multiome | 428 | |
| chr12:118,135,872–118,136,757 | 119.4 kb | Distal (>10kb) Multiome | 914 |
Genomic view of the RFC5 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.