This gene encodes a nuclear protein which interacts with the tumor suppressor retinoblastoma 1. The two interacting proteins are thought to act as a transcriptional repressor for promoters which are activated by the E2F1 transcription factor. This protein contains a Kruppel-associated box (KRAB), which is a transcriptional repressor motif. Read-through transcripts that include exons from the downstream gene LOC389458 are expressed from this locus. [provided by RefSeq, Mar 2011]
Transcription factors with Perturb-seq knockdown data for RBAK. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = RBAK upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of RBAK, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr7:4,775,021–4,775,903 | 270.3 kb | Distal (>10kb) Multiome | 860 | |
| chr7:4,791,775–4,793,216 | 253.5 kb | Distal (>10kb) Multiome | 359 | |
| chr7:4,798,157–4,798,757 | 247.5 kb | Distal (>10kb) Multiome | 349 | |
| chr7:4,845,690–4,846,237 | 199.9 kb | Distal (>10kb) Multiome | 165 | |
| chr7:4,882,152–4,884,513 | 162.1 kb | Distal (>10kb) Multiome | 585 | |
| chr7:4,973,785–4,974,732 | 71.8 kb | Distal (>10kb) Multiome | 674 | |
| chr7:5,042,157–5,042,509 | 3.3 kb | Proximal (<10kb) | 4 | |
| chr7:5,045,501–5,046,448 | 32 bp | At TSS Multiome | 594 | |
| chr7:5,189,698–5,190,896 | 144.3 kb | Distal (>10kb) Multiome | 725 | |
| chr7:5,240,214–5,240,895 | 194.6 kb | Distal (>10kb) Multiome | 613 | |
| chr7:5,296,049–5,297,455 | 250.9 kb | Distal (>10kb) Multiome | 191 |
Genomic view of the RBAK locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.