The RAS oncogene (MIM 190020) is mutated in nearly one-third of all human cancers. Members of the RAS superfamily are plasma membrane GTP-binding proteins that modulate intracellular signal transduction pathways. A subfamily of RAS effectors, including RASSF3, share a RAS association (RA) domain.[supplied by OMIM, Jul 2003]
Transcription factors with Perturb-seq knockdown data for RASSF3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = RASSF3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of RASSF3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:64,390,059–64,390,977 | 219.9 kb | Distal (>10kb) Multiome | 268 | |
| chr12:64,403,794–64,405,423 | 206.1 kb | Distal (>10kb) Multiome | 1007 | |
| chr12:64,451,663–64,452,624 | 158.4 kb | Distal (>10kb) Multiome | 977 | |
| chr12:64,543,521–64,544,182 | 66.7 kb | Distal (>10kb) Multiome | 54 | |
| chr12:64,608,914–64,611,341 | 113 bp | At TSS Multiome | 922 | |
| chr12:64,681,872–64,683,616 | 72.4 kb | Distal (>10kb) Multiome HiCAR | 397 | |
| chr12:64,758,714–64,760,174 | 148.9 kb | Distal (>10kb) Multiome | 809 | |
| chr12:64,780,224–64,781,694 | 170.3 kb | Distal (>10kb) Multiome | 599 | |
| chr12:64,824,273–64,825,855 | 214.2 kb | Distal (>10kb) Multiome | 351 |
Genomic view of the RASSF3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.