This intronless gene belongs to a family of RAS-related genes. The proteins encoded by these genes share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between the RAP and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. Evidence suggests that this protein may be polyisoprenylated and palmitoylated. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for RAP2B. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = RAP2B upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of RAP2B, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr3:153,062,044–153,062,830 | 99.9 kb | Distal (>10kb) Multiome | 111 | |
| chr3:153,078,635–153,079,723 | 83.1 kb | Distal (>10kb) Multiome | 228 | |
| chr3:153,160,269–153,163,526 | 887 bp | At TSS Multiome | 1217 | |
| chr3:153,166,306–153,166,593 | 4.1 kb | Proximal (<10kb) | 182 | |
| chr3:153,615,729–153,616,587 | 453.9 kb | Distal (>10kb) Multiome HiCAR | 140 | |
| chr3:153,640,205–153,641,766 | 478.7 kb | Distal (>10kb) Multiome HiCAR | 236 |
Genomic view of the RAP2B locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.